Inventors:
Chanchal Kumar Ghosh - West Chester OH
Michael Eugene Burns - Hamilton OH
David Neil DiGiulio - Hunt Valley MD
Edward Eugene Getty - Cincinnati OH
Richard Timothy Hartshorn - Newcastle Upon the Tyne, GB
Alan David Willey - Cincinnati OH
Philip F. Brode - Cincinnati OH
Bobby L. Barnett - Cincinnati OH
Donn N. Rubingh - Cincinnati OH
Assignee:
The Procter & Gamble Company - Cincinnati OH
International Classification:
C11D 3386
C11D 3395
C11D 328
Abstract:
The invention herein provides bleaching compositions comprising a protease enzyme which is a carbonyl hydrolase variant having an amino acid sequence not found in nature, which is derived by replacement of a plurality of amino acid residues of a precursor carbonyl hydrolase with different amino acids, wherein said plurality of amino acid residues replaced in the precursor enzyme correspond to position +76 in combination with one or more of the following residues: +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265, and/or +274, where the numbered positions corresponds to naturally-occurring subtilisin from Bacillus amyloliquefaciens or to equivalent amino acid residues in other carbonyl hydrolases or subtilisins (such as Bacillus lentus subtilisin) and a bleaching agent.