Sharon Baldwin

2009004, Feb 19, 2009

Aug 15, 2008

12/192762

Eva R. Birnbaum - Los Alamos NM, US
Benjamin P. Warner - Los Alamos NM, US
Sharon M. Baldwin - Santa Fe NM, US
Jennifer A. Berger - Los Alamos NM, US
Rebecca L.E. Miller - Los Alamos NM, US

Caldera Pharmaceuticals, Inc. - Los Alamos NM

G01N 23/223

378 45

The present invention includes an apparatus for preparing samples for measurement by x-ray fluorescence spectrometry. The apparatus comprises a plate having one or more holes passing through the plate. The holes are covered by a film on one side of the plate. The holes are less than 500 micrometers across in one dimension where the film covers the holes. The film is translucent to x-rays. The present invention also includes an apparatus for preparing samples for measurement by x-ray fluorescence spectrometry. The apparatus comprises a plate having one or more holes passing through the plate. The holes are covered on one side of the plate by a detachable cover forming a water-tight seal against the plate. The cover is substantially free of the elements osmium, yttrium, iridium, phosphorus, zirconium, platinum, gold, niobium, mercury, thallium, molybdenum, sulfur, lead, bismuth, technetium, ruthenium, chlorine, rhodium, palladium, argon, silver, and thorium. The holes are less than about 500 micrometers across in one dimension where the cover covers the holes. The present invention also includes a method for preparing samples for measurement by x-ray fluorescence spectrometry. The method comprises providing a solution of with less than 10 micromolar solute and a volume of between about 2 microliters and about 2 milliliters. The solution is concentrated and analyzed using x-ray fluorescence spectrometry.

2009008, Apr 2, 2009

Sep 26, 2008

12/239459

Eva R. Birnbaum - Los Alamos NM, US
Benjamin P. Warner - Los Alamos NM, US
Sharon M. Baldwin - Santa Fe NM, US
Jennifer A. Berger - Los Alamos NM, US
Michael N. Harris - Los Alamos NM, US
Rebecca L.E. Miller - Los Alamos NM, US

Caldera Pharmaceuticals, Inc. - Los Alamos NM

G01N 33/00
G01N 21/64

436 86, 436172, 436128, 436106

The present invention includes a method for analyzing reactions. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The solution further includes at least one controller chemical that affects the reaction between the donor chemical and the acceptor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The donor chemical includes a functional group selected from ester, anhydride, imide, acyl halide, and amide. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Yet another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. An additional analytical method is also used to measure either the acceptor product or the donor chemical.

2015030, Oct 29, 2015

Apr 22, 2015

14/693094

- Cambridge MA, US
Andrew T. Koppisch - Flagstaff AZ, US
Sharon M. Baldwin - Santa Fe NM, US
Benjamin P. Warner - Los Alamos NM, US
T. Mark McCleskey - Los Alamos NM, US
Jennifer A. Berger - Los Alamos NM, US
Jeffrey J. Stewart - Cary NC, US
Michael N. Harris - Los Alamos NM, US
Anthony K. Burrell - Naperville IL, US

G01N 33/566

X-ray fluorescence (XRF) spectrometry has been used for detecting binding events and measuring binding selectivities between chemicals and receptors. XRF may also be used for estimating the therapeutic index of a chemical. For estimating the binding selectivities of a chemical versus chemical analogs, for measuring post translational modification of proteins, and for drug manufacturing.

2008022, Sep 11, 2008

Oct 10, 2007

11/974156

Eva R. Birnbaum - Los Alamos NM, US
Andrew T. Koppisch - Flagstaff AZ, US
Sharon M. Baldwin - Santa Fe NM, US
Benjamin P. Warner - Los Alamos NM, US
T. Mark McCleskey - Los Alamos NM, US
Jeffrey Joseph Stewart - Los Alamos NM, US
Jennifer A. Berger - Los Alamos NM, US
Michael N. Harris - Los Alamos NM, US
Anthony K. Burrell - Los Alamos NM, US

G01N 33/53
G01N 21/76
G01N 33/68
G01N 23/223

435 71, 436501, 436172, 436 86, 378 45

X-ray fluorescence (XRF) spectrometry has been used for detecting binding events and measuring binding selectivities between chemicals and receptors. XRF may also be used for estimating the therapeutic index of a chemical, for estimating the binding selectivity of a chemical versus chemical analogs, for measuring post-translational modifications of proteins, and for drug manufacturing.

2013003, Feb 7, 2013

Aug 6, 2012

13/567613

Eva R. Birnbaum - Los Alamos NM, US
Benjamin P. Warner - Los Alamos NM, US
Sharon M. Baldwin - Santa Fe NM, US
Jennifer A. Berger - Los Alamos NM, US
Rebecca L.E. Miller - Los Alamos NM, US

Caldera Pharmaceuticals, Inc. - Los Almos NM

G01N 1/28
G01N 23/223

378 45, 378208

An apparatus for preparing samples for measurement by x-ray fluorescence spectrometry. A plate has one or more holes passing through the plate. The holes are covered by a film on one side of the plate. The holes are less than 500 micrometers across in one dimension where the film covers the holes. The film is translucent to x-rays.

2016020, Jul 14, 2016

Feb 25, 2016

15/052914

- Durham NC, US
Benjamin P. Warner - Los Alamos NM, US
Sharon M. Baldwin - Santa Fe NM, US
Jennifer A. Berger - Los Alamos NM, US
Michael N. Harris - Los Alamos NM, US
Rebecca L.E. Miller - Los Alamos NM, US

C12Q 1/48
C12Q 1/37
G01N 33/20
G01N 21/75

The present invention includes a method for analyzing reactions. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The solution further includes at least one controller chemical that affects the reaction between the donor chemical and the acceptor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The donor chemical includes a functional group selected from ester, anhydride, imide, acyl halide, and amide. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Yet another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. An additional analytical method is also used to measure either the acceptor product or the donor chemical.

2018023, Aug 23, 2018

Apr 24, 2018

15/961480

- DURHAM NC, US
Benjamin P. Warner - Los Alamos NM, US
Sharon M. Baldwin - Santa Fe NM, US
Jennifer A. Berger - Los Alamos NM, US
Michael N. Harris - Los Alamos NM, US
Rebecca L.E. Miller - Los Alamos NM, US

C12Q 1/48
G01N 33/68
C12Q 1/37
G01N 21/75
G01N 23/223
G01N 33/20

The present invention includes a method for analyzing reactions. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The solution further includes at least one controller chemical that affects the reaction between the donor chemical and the acceptor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The donor chemical includes a functional group selected from ester, anhydride, imide, acyl halide, and amide. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Yet another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. An additional analytical method is also used to measure either the acceptor product or the donor chemical.

2020015, May 21, 2020

Jan 23, 2020

16/750144

- Durham NC, US
Benjamin P. WARNER - Los Alamos NM, US
Sharon M. BALDWIN - Santa Fe NM, US
Jennifer A. BERGER - Los Alamos NM, US
Michael N. HARRIS - Los Alamos NM, US
Rebecca L.E. MILLER - Los Alamos NM, US

C12Q 1/48
G01N 33/68
G01N 23/223
G01N 33/20
C12Q 1/37
G01N 21/75

The present invention includes a method for analyzing reactions. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The solution further includes at least one controller chemical that affects the reaction between the donor chemical and the acceptor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The donor chemical includes a functional group selected from ester, anhydride, imide, acyl halide, and amide. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Yet another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. An additional analytical method is also used to measure either the acceptor product or the donor chemical.